›› 2012, Vol. 24 ›› Issue (4): 245-248，.doi: 10.3969/j.issn.1004-616x.2012.04.001

• 论著 •     Next Articles

Analysis of PIK3CA gene mutations in Chinese colorectal cancer

CHEN Xi1;ZHANG Tong-tong;LIANG Jian-wei;SHANG Li; HUO Yan-qiu;XU Xin;ZHOU Zhi-xiang;WANG Ming-rong;ZHANG Yu;JIA Xue-mei   

  1. (1. Department of Human Anatomy and Histology, Institute of Basic Research, Anhui Medical University, Hefei 230032, Anhui; 2. State Key Laboratory of Molecular Oncology, Cancer Institute of Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021; 3. Abdominal Surgery Department of Cancer Hospital, Academy of Medical Sciences, Beijing 100021, China)
  • Received:2012-03-07 Revised:2012-06-07 Online:2012-07-30 Published:2012-07-30
  • Contact: ZHANG Yu；JIA Xue-mei

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Abstract: OBJECTIVE: PIK3CA gene encodes the p110α catalytic subunit of PI3K, and its oncogenic mutation is involved in tumorigenesis of colorectal cancer (CRC) through activating of PI3K pathway. Frequent mutation of the PIK3CA gene in Western CRC has been reported; however, the mutations status of the gene in Chinese CRC is unclear. The aim of the study was to evaluate the mutation status of PIK3CA gene in Chinese patients with CRC. METHODS：PIK3CA gene mutations were analyzed in 79 colorectal tumors by PCR and DNA sequencing. RESULTS：PIK3CA gene mutations were detected in 8.9% (7/79) of tumor samples 6.3% (5/79) and 2.5% (2/79) of point mutations were found in exon 9 and exon 20, respectively. The distribution pattern of PIK3CA gene mutations was consistent with previous studies. CONCLUSION：A subset of Chinese CRC with PIK3CA gene mutations was identified in the study. Oncogenic mutation of PIK3CA gene may contribute to PI3K pathway activation in the corresponding tumors.

Key words: colorectal cancer, PIK3CA, point mutation, PI3K pathway